Účinky nízkovýkonného laserového záření na pohyb buněk protozoí
| Title in English | Effects of the low-powered laser irradiation on the movement of protozoan cells |
|---|---|
| Authors | |
| Year of publication | 2004 |
| Type | Article in Proceedings |
| Conference | XXVII. Dny lékařské biofyziky, Sborník abstrakt |
| MU Faculty or unit | |
| Citation | |
| Field | Biophysics |
| Keywords | Therapeutic laser; protozoan movement |
| Description | Two different protozoan species, Euglena gracilis and Tetrahymena thermophila, were used to study changes in locomotion behavior in response to low-power lasers. The cells were irradiated at 830 nm and 650 nm generated by a semiconductor laser (99 J/cm2, 360mW) and a laser pointer (0.75 J/cm2, 5mW), respectively, and their locomotion was recorded by a TV camera and analyzed by computer software. Exposure to laser light, regardless of the wavelength, resulted in increased cell velocity in both species (P<0.001). Exposure to 650 nm produced an equal increase in median cell velocity in both Euglena gracilis (19.0 % ) and Tetrahymena thermophila (18.2 %) and some increase persisted in the post-irradiation 30-second period. Irradiation by 830 nm resulted in a markedly higher response in Tetrahymena (29.4 %) than in Euglena (15.2%) and the two median values remained increased after irradiation was discontinued. Different reactions found in the species studied and some mechanisms underlying the response of cells to radiation are discussed. |
| Related projects: |