RYCHLÉ HPLC STANOVENÍ FENOTYPU CYP 1A, 2C A 3A Z PERFUZÁTU IZOLOVANÝCH JATER
| Title in English | RAPID HPLC DETERMINATION OF PHENOTYPE CYP 1A, 2C AND 3A FROM THE PERFUSATE MEDIUM AFTER ISOLATED RAT LIVER PERFUSION |
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| Authors | |
| Year of publication | 2005 |
| Type | Article in Periodical |
| Magazine / Source | Československá fyziologie |
| MU Faculty or unit | |
| Citation | |
| Field | Pharmacology and pharmaceutical chemistry |
| Keywords | phenacetine; midazolam; tolbutamide; CYP1A; CYP2C; CYP3A |
| Description | HPLC methods trace the markers of the metabolic activity of CYP3A1/2 (midazolam and its three hydroxymetabolites: 1-hydroxymidazolam, 1,4-dihydroxymidazolam, and 4-hydroxymidzolam), CYP2C6(tolbutamide and its metabolite hydroxytolbutamide) or CYP1A2(phenacetine and its metabolite paracetamol). Proposed methods are simple, rapid, sensitive and applicable for modeling and description of possible pharmacological interactions on rat CYP3A1/2, CYP2C6 or CYP1A2, respectively. |
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