Immunohistochemical detection of stromal cell-derived factor (SDF-1) and its CXCR4 receptor in the rat dorsal root ganglia after nerve ligature
| Authors | |
|---|---|
| Year of publication | 2006 |
| Type | Article in Proceedings |
| Conference | FENS Abstr. |
| MU Faculty or unit | |
| Citation | |
| Field | Neurology, neurosurgery, neurosciences |
| Keywords | chemokine-dorsal root ganglia-neuropathic pain |
| Description | Stromal cell-derived factor SDF-1 (chemokine ligand CXCL12) belongs to chemokines, small secreted proteins whose effects are mediated by stimulating G-protein-coupled receptors. The chemokine and its receptor CXCR4 are essential for neuro–immune homeostatic functions playing a crucial role not only in the regulation of cellular movement, proliferation, plasticity and survival of the neurons and lymphocytes, but also in pain signaling. The primary sensory neurons of the dorsal root ganglia (DRG) play a key role in neuropathic pain, which has been investigated using rat peripheral nerve injury models including unilateral sciatic and spinal nerve ligature. The goal of present study was to demonstrate immunohistochemical distribution of SDF-1 and CXCR4 in the naive DRG and those following nerve ligatures. The SDF-1 and CXCR4 immunofluorescence found in the DRG affected by unilateral nerve ligature was compared with the contralateral ones removed from operated animals and with those removed from naive rats. The results revealed dynamics in the expression of the chemokine and its receptor suggesting their possible role in the transmission of cellular and molecular changes to the DRG contralateral from the nerve injury. |
| Related projects: |