HPLC determination of midazolam and its three hydroxy metabolites in perfusion medium and plasma from rats

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Authors	JUŘICA Jan DOSTÁLEK Miroslav KONEČNÝ Jiří GLATZ Zdeněk HADAŠOVÁ Eva TOMANDL Josef
Year of publication	2007
Type	Article in Periodical
Magazine / Source	Journal of Chromatography B
MU Faculty or unit	Faculty of Science
Citation
Web	http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6X0P-4N4J320-4&_user=835458&_coverDate=06%2F01%2F2007&_alid=584152625&_rdoc=1&_fmt=summary&_orig=search&_cdi=7220&_sort=d&_st=4&_docanchor=&_ct=44&_acct=C000045159&_version=1&_urlVersion=0&_userid
Field	Biochemistry
Keywords	cytochrome P450; midazolam; 1'-hydroxymidazolam; 4-hydroxymidazolam; 1'4-dihydroxymidazolam; rat; perfusion; plasma; HPLC
Description	A new, simple, rapid, sensitive, and repeatable isocratic reverse-phase HPLC method was developed and validated for simultaneous determination of midazolam and its main three hydroxylated metabolites, i.e. 1' hydroxymidazolam, 4-hydroxymidazolam, and 1',4-dihydroxymidazolam in rat liver perfusate and also plasma. Diazepam was used as an internal standard to ensure precision and accuracy of this method. This method is applicable for modeling and description of possible pharmacological interactions on rat (CYP3A1/2) or human (CYP3A4/5) cytochrome P450 enzymes.
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