Neuroinflammatory reaction of DRG following unilateral nerve injury is bilaterally spread alongside neuroaxis

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Year of publication 2010
Type Conference abstract
MU Faculty or unit

Faculty of Medicine

Description Wallerian degeneration following mechanical injury of the peripheral nerve has a significant inflammatory component (neuroinflammation), primarily due to the production of variety of mediators including cytokines. Compelling evidence indicates that neuropathic pain induced by nerve injury is related to changes in the dorsal root ganglia (DRG) containing bodies of the primary afferent neurons. Unilateral chronic constriction injury (CCI) of the sciatic nerve was performed aseptically under deep anesthesia in sixty fore rats. Neuropathic pain induction was tested by withdrawal threshold of mechanoallodynia and thermal hyperalgesia. Immunohistochemical detection of TNFa, IL-6, and their receptors as well as IL-10 was investigated in both ipsilateral and contralateral lumbar (L4-5) and cervical (C7-8) DRG following CCI for 1, 3, 7 and 14 days. In addition, Western blot and ELISA were used for quantitative analyses and in situ hybridization to prove synthesis of cytokines. We have demonstrated that bilateral changes of cytokines in DRG are not limited only to segments associated with injured nerve but also spread alongside neuroaxis to non-associated DRG. We hypothesize that signaling for extension of neuroinflammation could be based on cytokine-cytokine interactions, and/or prostanoids and complement proteins can be involved. However, it remains to confirm what signals are responsible for propagation of neuroinflammation into DRG far from nerve injury.
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