Sample preparation methods for MALDI-MS profiling of bacteria

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Authors	ŠEDO Ondrej SEDLÁČEK Ivo ZDRÁHAL Zbyněk
Year of publication	2011
Type	Article in Periodical
Magazine / Source	Mass Spectrometry Reviews
MU Faculty or unit	Central European Institute of Technology
Citation
Doi	http://dx.doi.org/10.1002/mas.20287
Field	Analytic chemistry
Keywords	MALDI-MS; bacteria profiling; microorganism identification
Description	Direct matrix-assisted laser desorption/ionization mass spec- trometry (MALDI-MS) bacterial cell or lysate analysis appears to meet all the criteria required for a rapid and reliable analytical microorganism identification and taxonomical classification tool. Few-minute analytical procedure providing information extending up to sub-species level underlines the potential of the MALDI-MS profiling in comparison with other methods employed in the field. However, the quality of MALDI-MS profiles and consequently the performance of the method are influenced by numerous factors, which involve particular steps of the sample preparation procedure. This review is aimed at advances in development and optimization of the MALDI-MS profiling methodology. Approaches improving the quality of the MALDI-MS profiles and universal feasibility of the method are discussed.
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