Selectivity among Two Lectins: Probing the Effect of Topology, Multivalency and Flexibility of “Clicked” Multivalent Glycoclusters

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Authors	CECIONI Samy FAURE Sophie DARBOST Ulrich BONNAMOUR Isabelle PARROT-LOPEZ Helene ROY Olivier TAILLEFUMIER Claude WIMMEROVÁ Michaela PRALY Jean-Pierre IMBERTY Anne VIDAL Sebastien
Year of publication	2011
Type	Article in Periodical
Magazine / Source	Chemistry - A European Journal
MU Faculty or unit	Central European Institute of Technology
Citation
Doi	http://dx.doi.org/10.1002/chem.201002635
Field	Biophysics
Keywords	lectin; glycocluster;SPR;ITC
Description	We have designed a series of multivalent galactosylated glycoconjugates and studied their binding properties towards two lectins, from plant and bacterial origins, to determine their potential selectivity. The synthesis was achieved through copper(I)-catalysed azide-alkyne cycloaddition (CuAAC) under microwave activation between propargylated multivalent scaffolds and an azido-functionalised carbohydrate derivative. The interactions of two galactose-binding lectins from Pseudomonas aeruginosa (PA-IL) and Erythrina cristagalli (ECA) with the synthesized glycoclusters were studied by hemagglutination inhibition assays (HIA), surface plasmon resonance (SPR) and isothermal titration microcalorimetry (ITC). The results obtained illustrate the influence of the scaffold's geometry on the affinity towards the lectin and also on the relative potency in comparison with a monovalent galactoside reference probe.
Related projects:	Proteins in metabolism and interaction of organisms with the environment Program developing interdisciplinary research POtential for the STudies of BIOMolecular INteractions Design of Carbohydrates and Glycomimetics as Antibacterial and Antiviral Drugs