Streptavidin-modified magnetic poly(2-hydroxyethyl methacrylate-co-g-lycidyl methacrylate) microspheres for selective isolation of bacterial DNA.

• Authors: HORÁK Daniel; ŠPANOVÁ Alena; TVRDÍKOVÁ Jana; RITTICH Bohuslav
• Year of publication: 2011
• Type: Article in Periodical
• Magazine / Source: European Polymer Journal
• MU Faculty or unit: Faculty of Science
• Doi: http://dx.doi.org/10.1016/j.eurpolymj.2011.02.007
• Field: Macromolecular chemistry
• Keywords: Glycidyl methacrylate; 2-hydroxyethyl methacrylate; Streptavidin; Biotin; Magnetic Microspheres; DNA
• Description: The dispersion copolymerization of 2-hydroxyethyl methacrylate (HEMA) with glycidyl methacrylate (GMA) in a toluene/2-methylpropan-1-ol mixture in the presence of Fe3O4 nanoparticles coated with oleic acid, produced monodisperse magnetic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-GMA)) microspheres. Oxirane groups of the microspheres were ammonolyzed and then functionalized with streptavidin using cyanuric chloride. The final product contained 0.67 mg of streptavidin per gram of wet magnetic P(HEMA-GMA) microspheres. The microspheres were characterized by elemental analysis, scanning electron microscopy, IR, UV–VIS and atomic absorption spectroscopy The streptavidin-modified magnetic P(HEMA-GMA) microspheres were used for immobilization of biotinylated DNA and subsequent selective isolation of target DNA from complex samples using DNA/DNA hybridisation.

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