Endonuclease G interacts with histone H2B and DNA topoisomerase II alpha during apoptosis


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Year of publication 2012
Type Article in Periodical
Magazine / Source Mol Cell Biochem
MU Faculty or unit

Faculty of Informatics

Web http://link.springer.com/article/10.1007%2Fs11010-011-1182-x
Doi http://dx.doi.org/10.1007/s11010-011-1182-x
Field Genetics and molecular biology
Keywords endonuclease G; DNA topoisomerase II alpha; histone H2B; FRET; apoptosis
Attached files
Description During some apoptotic pathways, proteins apoptosis-inducing factor (AIF) and endonuclease G (EndoG) are released from the mitochondria and they translocate into the cell nuclei, where they probably participate in chromatin degradation together with other nuclear proteins. Exact mechanism of EndoG activity in cell nucleus is still unknown. We conducted a living-cell confocal fluorescence microscopy followed by an image analysis of fluorescence resonance energy transfer (FRET) to analyze the possibility of protein interactions of EndoG with histone H2B and human DNA topoisomerase II alpha (TOPO2a). Our results show that EndoG interacts with both these proteins during apoptotic cell death. Therefore, we can conclude that EndoG and TOPO2a may actively participate in apoptotic chromatin degradation. The possible existence of a degradation complex consisting of EndoG and TOPO2a and possibly other proteins like AIF and cyclophilin A have yet to be investigated.
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