New role for L-arginine in regulation of inducible nitric-oxide-synthase-derived superoxide anion production in raw 264.7 macrophages.

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Authors	PEKAROVÁ Michaela LOJEK Antonín MARTIŠKOVÁ Hana VAŠÍČEK Ondřej BINÓ Lucia KLINKE Anna LAU Denise KUCHTA Radek KADLEC Jaroslav VRBA Radimír KUBALA Lukáš
Year of publication	2011
Type	Article in Periodical
Magazine / Source	The Scientific World Journal
MU Faculty or unit	Faculty of Science
Citation
Web	http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3246759/?tool=pubmed
Doi	http://dx.doi.org/10.1100/2011/321979
Field	Ecology
Keywords	Macrophages; L-arginine; inducible nitric oxide synthase; superoxide anion; NO
Description	Dietary supplementation with L-arginine was shown to improve immune responses in various inflammatory models. However, the molecular mechanisms underlying L-arginine effects on immune cells remain unrecognized. Herein, we tested the hypothesis that a limitation of L-arginine could lead to the uncoupled state of murine macrophage inducible nitric oxide synthase and, therefore, increase inducible nitric-oxide-synthase-derived superoxide anion formation. Importantly, we demonstrated that L-arginine dose- and time dependently potentiated superoxide anion production in bacterial endotoxin-stimulated macrophages, although it did not influence NADPH oxidase expression and activity.