Characterization of autologous T cell clones reactive against metastatic melanoma

Investor logo
Project Identification
Project Period
1/2004 - 12/2006
Investor / Pogramme / Project type
Czech Science Foundation
MU Faculty or unit
Faculty of Medicine
T cell, clone, melanoma

The objective of this proposal is to ident´fy and characterize autologous T cells that are able to specifically destroy tumor cells from patients with metastatic melanoma. Melanoma cell apoptotic bodies-pulsed dentritic cells will be used to activate autologous T cells. Melanoma-specific T cells will be activated andsorted out by flow cytometer. Clonality of these activated T cells will be studied using cDNA library and direct sequenciong of T cell receptor beta genes. Melanoma-specific T cell clones will be identified, quantified, expanded, tested for cytotoxicity against original melanoma cells, and potentially use in future clicical studies.


Total number of publications: 5

You are running an old browser version. We recommend updating your browser to its latest version.

More info