Foton-upkonverzní značky pro mikrofluidní jednomolekulové imunostanovení proteinových biomarkerů
- Project Identification
- GA21-03156S
- Project Period
- 4/2021 - 3/2024
- Investor / Pogramme / Project type
-
Czech Science Foundation
- Standard Projects
- MU Faculty or unit
- Faculty of Science
- Keywords
- photon-upconversion; nanoparticles; immunochemistry; biomarkers; microfluidics; single-molecule methods
- Cooperating Organization
-
The Academy of Sciences of the Czech Republic
- Responsible person Mgr. Antonín Hlaváček, Ph.D.
The project proposes a new development of single-molecule immunoassays using the luminescence of photon-upconversion nanoparticles. This type of luminescence is characterized by excitation in the infrared region and emission of short-wavelength radiation (mostly visible). The low background of this type of luminescence simplifies the imaging and counting of individual nanoparticles; when used as a luminescent label, the counting of the single analyte molecules is possible. The innovation is based on using microfluidic chips for automation. Microfluidics decreases analysis time, reagent consumption, detection limits, and facilitates the analysis of microscopic samples (single-cell analysis). The methodology will be verified by the detection of known protein biomarkers: (A) prostate-specific antigen in blood serum and plasma and (B) protein biomarker p53 at the level of individual cells. The project aims to improve disease diagnostics and monitoring the course of disease treatment and advances the toolbox of cell and molecular biology.
Publications
Total number of publications: 16
2022
-
Bioconjugates of photon-upconversion nanoparticles for cancer biomarker detection and imaging
Nature Protocols, year: 2022, volume: 17, edition: April 2022, DOI
-
PMVEMA-coated upconverting nanoparticles for upconversion-linked immunoassay of cardiac troponin
Talanta, year: 2022, volume: 244, edition: 123400, DOI
-
Up and down the spectrum: upconversion nanocrystal and semiconductor material fused into a single nanocomposite
Light: Science & Applications, year: 2022, volume: 11, edition: 179, DOI
2021
-
Advanced Immunochemical Biosensing: From Label-Free to Single-Molecule Detection
Year: 2021, type:
-
Advanced Immunochemical Biosensors and Assays: From Label-Free to Single-Molecule Detection
Year: 2021, type:
-
Atomic force microscopy and surface plasmon resonance for real-time single-cell monitoring of bacteriophage-mediated lysis of bacteria
Nanoscale, year: 2021, volume: 13, edition: 31, DOI
-
Digital detection of subfemtomolar concentrations of prostate-specific antigen by single molecule immunosensing
Year: 2021, type: Conference abstract
-
Effect of Particle Size and Surface Chemistry of Photon-Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin
Advanced Healthcare Materials, year: 2021, volume: 10, edition: 18, DOI
-
Immunochemical sensing with photon-upconversion nanoparticles: From analog to digital format
Year: 2021, type:
-
Photon-upconversion nanoparticles as a background-free label in immunosensing and bioimaging
Year: 2021, type: Conference abstract