Xeno- and feeder-free derivation of two sex-discordant sibling lines of human embryonic stem cells

Authors

SOURALOVÁ Tereza HOLUBCOVÁ Zuzana KYJOVSKÁ Drahomíra HAMPL Aleš KOUTNÁ Irena

Year of publication 2021
Type Article in Periodical
Magazine / Source Stem Cell Research
Citation
Doi http://dx.doi.org/10.1016/j.scr.2021.102574
Description Human embryonic stem cells (hESCs) represent a virtually unlimited source of cells suitable for a variety of biomedical applications. However, a diminishing allogeneic background and undefined culture conditions are essential for developing robust and replicable protocols for differentiation experiments, disease modeling, and drug testing. Therefore, here we report the generation of the two sex-discordant sibling hESC lines, MUNIe008-A and MUNIe009-A, using the mechanical biopsy of vitrified-thawed embryos under xeno- and feeder-free conditions. The presented approach is applicable for deriving high-quality clinical-grade hESC lines for cell replacement therapies.
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