Imunomagnetická separace buněk z kostní dřeně u pacientů s mnohočetným myelomem stanovení delece 13q14 použitím interfázní fluorescenční in situ hybridizace

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Title in English Immunomagnetic separation of bone marrow cells in multiple myeloma patiens detection of 13q14 deletion using interphase fluorescence in situ hybridization
Authors

SMEJKALOVÁ Jana VRANOVÁ Vladimíra KOVÁŘOVÁ Lucie KUGLÍK Petr FILKOVÁ Hana HEINIGOVÁ Jana ADAM Zdeněk KREJČÍ Marta BÜCHLER Tomáš KALÁBOVÁ Vladimíra OLTOVÁ Alexandra VORLÍČEK Jiří PENKA Miroslav HÁJEK Roman

Year of publication 2005
Type Article in Periodical
Magazine / Source Klinická onkologie
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords Multiple myeloma; magnetic-activated cell separation; interphase fluorescence in situ hybridization; 13q14 deletion
Description Background: Chromosomal abnormalities, such as 13q deletion, are emerging as important prognostic factors in multiple myeloma. The availability of cytogenetic information has long been hampered by the low mitotic aktivity of the plasma cells. To obtain a direkt assessment of the myeloma cells patology in high purity cell population, we have used magnetics-activated cell sorting in combination with intephase fluorescence in situ hybridization. Design and subjekt: The study included 40 patients with multiple myeloma. Methods and results: We used chromosomal G-banding and interphase in situ hybridization on CD 138+ and CD 138- cells after selection by magnetic-activated cell separation. Hybridization was performed to detected the 13q14 deletion in unselected bone marrow cells and, in paralel, in CD 138+ enriched samples.. The 13q14 deletion was found in 10 of 40 (25%) of bone marrow samples without cell selection and in 25 of 40 (62,5%) of samples with CD 138+ enriched myeloma cells.Conclusion: Our results confirm that immunomagnetic selection of CD 138+ cells increases the probability of detection of the 13q14 deletion in bone marrow samples.
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