The text has been created and designed for one-semester English program of General medicine and Dentistry studies in the Faculty of Medicine, Masaryk University, Brno. The selection of topics emphasizes the biochemical processes and the metabolic conversions of compounds occurring in the human body. The text describes the most important biomolecules in the human tissues (saccharides, lipids, amino acids, proteins, nucleic acids), however, it also comprises essential facts from general chemistry (energy, equilibrium, kinetics, acids and bases, redox reactions, solutions, etc.), summarizes biologically important elements (macroelements, microelements), and treats main groups of organic compounds (alcohols, aldehydes, amines, carboxylic acids, heterocycles, etc.). Some special topics are also included like colloidal solutions, dispersions, osmotic pressure, Latin nomenclature, steroids, terpenes, glycoproteins, vitamins, selected toxic substances.