Analysis of B-cells and plasma cells in multiple myeloma

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Year of publication 2012
Type Conference abstract
MU Faculty or unit

Faculty of Medicine

Description Multiple myeloma (MM) is characterized by the presence of clonal plasma cells (PC) arising from malignant transformed B-cells. It is still not clear which stage of B-cell differentiation is responsible for the development of MM and for eventual relapse after treatment, so nowadays there is an effort to identify the source of myeloma-initiating cells. Aim: Analysis of the phenotypic profile and enumeration of B and PC subpopulations in MM patients. Patients and methods: Total of 38 newly diagnosed MM patients and 18 controls without MM were analysed. Results: There was found significantly lower % of the total CD19+ cells in MM than in control samples for both PB and BM (p <0.05). Numbers of transitional B-cells and naive B-cells were reduced in PB of MM when compared with controls (p <0.05). On the contrary, numbers of preGC, total memory and CD27- switched B-cells were increased in MM (p <0.05). No difference was found when compared % of plasmablasts in both groups. There were found similar results as reduction of naive B-cells and increase of total memory and CD27- switched B-cells in BM of MM (p <0.05). As expected, higher % of PC was found in MM mostly with abnormal phenotype CD19- (p <0.005). Conclusion: Polychromatic flow cytometry is capable to identify minimum of 10 B-cell and PC subpopulations. Reduction of CD19+ cells in MM should be mediated by an increased number of abnormal PCs. The enhancement of isotype-switched memory CD27+ and isotype-switched CD27- B-cell subpopulations in MM patients suggests that some of these could be a potential source of myeloma-initiating cells. Analysis on DNA level will be done.
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