IDENTIFICATION OF HUMAN MICROBIOME IN HARD-TO-HEAL WOUNDS BY MALDI-TOF MS AND SEQUENCING
| Authors | |
|---|---|
| Year of publication | 2014 |
| Type | Article in Proceedings |
| Conference | CECE 2014: 11th International Interdisciplinary Meeting on Bioanalysis |
| MU Faculty or unit | |
| Citation | |
| Web | http://www.ce-ce.org/CECE2014/CECE%202014%20proceedings_full.pdf |
| Field | Genetics and molecular biology |
| Keywords | HARD-TO-HEAL WOUNDS; MALDI-TOF MS; SEQUENCING |
| Description | This study is focused on the isolation, characterization and identification of the most abundant microorganisms in superficial wounds of patients (n = 50) suffering from bacterial infection. Primarily, the identification of the strains was carried out through Matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) based on comparison of protein profiles (2-30 kDa) with database. Sanger sequencing of 16S bacterial gene was used for confirmation if the score was < 2. |
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