IDENTIFICATION OF HUMAN MICROBIOME IN HARD-TO-HEAL WOUNDS BY MALDI-TOF MS AND SEQUENCING

Autoři	CHUDOBOVA Dagmar CIHALOVA Kristyna GURÁŇ Roman DOSTALOVA Simona SMERKOVA Kristyna VESELÝ Radek GUMULEC Jaromír HEGER Zbynek MASAŘÍK Michal ADAM Vojtěch KIZEK René
Rok publikování	2014
Druh	Článek ve sborníku
Konference	CECE 2014: 11th International Interdisciplinary Meeting on Bioanalysis
Fakulta / Pracoviště MU	Lékařská fakulta
Citace
www	http://www.ce-ce.org/CECE2014/CECE%202014%20proceedings_full.pdf
Obor	Genetika a molekulární biologie
Klíčová slova	HARD-TO-HEAL WOUNDS; MALDI-TOF MS; SEQUENCING
Popis	This study is focused on the isolation, characterization and identification of the most abundant microorganisms in superficial wounds of patients (n = 50) suffering from bacterial infection. Primarily, the identification of the strains was carried out through Matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) based on comparison of protein profiles (2-30 kDa) with database. Sanger sequencing of 16S bacterial gene was used for confirmation if the score was < 2.
Související projekty:	CEITEC - středoevropský technologický institut