The Root Growth-Regulating Brevicompanine Natural Products Modulate the Plant Circadian Clock

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Authors	MONTAIGU A. OELJEKLAUS A. KRAHN J. SULIMAN J.H. HALDER M.N.S. ANSORENA V. de NICKEL E. SCHLICHT S. PLIHAL M. KUBIASOVA O. RADOVÁ Lenka KRACHER L. TOTH B. KASCHANI R. COUPLAND F. KOMBRINK G. KAISER E.
Year of publication	2017
Type	Article in Periodical
Magazine / Source	ACS CHEMICAL BIOLOGY
MU Faculty or unit	Central European Institute of Technology
Citation
Web	http://pubs.acs.org/doi/abs/10.1021/acschembio.6b00978
Doi	http://dx.doi.org/10.1021/acschembio.6b00978
Field	Biochemistry
Keywords	ARABIDOPSIS-THALIANA; CHEMICAL BIOLOGY; GENES; EXPRESSION; OPPORTUNITIES; SYSTEM; AUXIN; TIME
Description	Plant growth regulating properties of brevicompanines (Brvs), natural products of the fungus Penicillium brevicompactum, have been known for several years, but further investigations into the molecular mechanism of their bioactivity have not been performed. Following chemical synthesis of brevicompanine derivatives, we studied their activity in the model plant Arabidopsis by a combination of plant growth assays, transcriptional profiling, and numerous additional bioassays. These studies demonstrated that brevicompanines cause transcriptional misregulation of core components of the circadian clock, whereas other biological read-outs were not affected. Brevicompanines thus represent promising chemical tools for investigating the regulation of the plant circadian clock. In addition, our study also illustrates the potential of an unbiased -omics-based characterization of bioactive compounds for identifying the often cryptic modes of action of small molecules.