Orální bakteriom u pacientů s dlaždicobuněčným karcinomem dle stavu jejich dentice: pilotní studie

Title in English Oral bacterioma in patients with squamous cell carcinoma based on their gingival status: a pilot study


Year of publication 2023
Type Conference abstract
MU Faculty or unit

Faculty of Medicine

Description Introduction: Oral squamous cell carcinoma (OSCC) is the most common malignancy in the head and neck region. Factors influencing the etiopathogenesis of OSCC include oral dysbiosis and malhygiene. Compared to dentate people, people with complete tooth loss (similar to people with good oral hygiene) have less oral bacteriomyoma diversion. In studies of controls and cases, significantly greater bacterial diversity was observed on the tumor surface in patients with OSCC compared to healthy oral mucosa samples in controls. The aim of this pilot study was to analyze and compare the bacteriome on the tumor surface and from the dental plaque and to determine whether the bacterial colonization of the tumor surface differs according to the status of the dentition in patients with OSCC. Methods: Thirty-two patients with a histopathologically confirmed diagnosis of OSCC were divided into 3 groups according to the condition of their dentition into edentulous, under-edentulous (<16 teeth), and over-edentulous (?16 teeth). In all of them, a swab was taken from the tumor surface and in the dentate ones, in addition, a dental plaque swab was taken. Microbial DNA was isolated using the QIAamp DNA Mini Kit (QIAGEN), and the 16S rRNA hypervariable region was amplified and a library was prepared for next-generation sequencing on an Illumina MiSeq instrument. Results: The alpha diversity of the bacteriome was comparable in matrices obtained from tumor and dental plaque swabs (p > 0.05). In both types of samples, Streptococcus, Fusobacterium and Porphyromonas were among the most abundant genera. We found a difference in bacterial diversity (according to ASV) from the tumor surface when comparing edentulous and low-toothed patients (<16 teeth; p < 0.05); we observed only a similar trend in the more toothed patients (p > 0.05). The relative abundance of the genus Dialister sp. on the tumor surface was higher in the low-toothed compared to the more toothed patients (p < 0.05), whereas there was no difference between the no- and low-toothed patients (p > 0.05). The level of oral hygiene and the number of ASV in the dental plaque samples were similar between the toothed patient groups (p > 0.05). Summary: The results of our pilot study showed that completely edentulous patients had lower bacterial diversity on the tumor surface than the edentulous ones. This supports the hypothesis that the oral bacteriome in OSCC patients is influenced by the condition of their dentition.
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