Neutrophil and eosinophil granulocyte immunophenotyping and a burst test



Year of publication 2023
Type Conference abstract
MU Faculty or unit

Faculty of Medicine

Description Neutrophils and eosinophils are among the largest granulocytes observed in clinical flow cytometry. They are commonly gated from the basic FSC vs SSC dot plot based on their size and granularity, or by using the pan-leukocyte antigen CD45 and SSC. Routine laboratories usually measure only the relative numbers of neutrophils or eosinophils. In terms of functional tests, these include the test of oxidative burst in neutrophils to rule out the diagnosis of chronic granulomatosis, or the test for the presence of myeloperoxidase (MPO) in case of suspected immunodeficiency of MPO. Inflammation or bone marrow disorders are the most common causes of increased neutrophil representation and an increased presence of immature neutrophils in peripheral blood (PB). Eosinophil granulocytes are commonly increased in PB of various diseases including allergic eosinophilic asthma, allergic eczema, parasite infections or immunodeficiencies like Omenn syndrome and hyper IgE syndrome. Our study aimed to perform a functional test of the oxidative burst of neutrophils and eosinophils with their simultaneous phenotyping in patients with eosinophilic asthma and patients after hip surgery. The results showed that immature neutrophils and eosinophils have a reduced oxidative burst capacity compared to their mature forms. Immunophenotyping of neutrophils and eosinophils allows the recognition of their mature and immature forms, which differ in their functional properties. Our findings could help in the interpretation of findings in patients with partially reduced oxidative burst.
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