Antiradical activity of aporhine alkaloids



Year of publication 2002
Type Article in Proceedings
MU Faculty or unit

Faculty of Medicine

Keywords aporphine alkaloids; antioxidative; DPPH; HPLC-EC
Description The aim of this work was to determine the antiradical ability using DPPH assay and the redox properties (oxidative potential) using HPLC-EC. Antiradical activity was related with a presence, number and position of phenolic groups. The presence of free electron pair on the nitrogen atom also participated in stronger antiradical activity, as was described for the inhibition of lipid peroxidation. The antiradical activity of corytuberine (IC50= 11,4 uM), the strongest antioxidant among the aporphines tested, was comparable with the activity of ascorbic acid (IC50= 10,3 uM). One mole of corytuberine reacted approximately with 2 moles of DPPH radical, similarly as ascorbic acid. Oxidative potentials characterising the ability of aporhines to be oxidised were in agreement with results obtained using DPPH. Aporphines glaucine, magnoflorine, menisperine, isocorydine, bulbocapnine, isothebaine, corydine with lower oxidative potential (<450mV) are a good scavengers of DPPH. These findings indicate that HPLC-EC method together with DPPH method is a good tool to evaluate the antiradical activity of compounds of natural origin.

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