Impedance analysis of DNA and DNA-drug interactions on thin mercury film electrodes.
| Authors | |
|---|---|
| Year of publication | 2002 |
| Type | Article in Periodical |
| Magazine / Source | Critical Reviews in Analytical Chemistry |
| MU Faculty or unit | |
| Citation | |
| Field | Biophysics |
| Keywords | electrochemical impedance spectroscopy; EIS; DNA adsorption; echinomycin; DNA-echinomycin interaction; mercury film electrodes; |
| Description | Electrochemical behaviour of echinomycin, DNA and DNA-echinomycin complex at the mercury film electrodes (MFE) was studied by measurement of the impedance of the electrode double layer (dependence of the differential capacitance C on the electrode potential, C-E curves, and dependence of the impedance Z on ferquency - electrochemical impedance spectroscopy, EIS). Mercury film was plated either on a glassy carbonelectrode (GCE) or pyrolytic graphite electrode (PGE). The capacitance measurement showed that echinomycin forms the pseudocapacitance redox peak around potential -0.53 V at the MFE.Interaction of single stranded (ss) and double stranded (ds) DNA with echinomycin at DNA-modified electrode surface was studied using EIS with adsorptive transfer method. Pseudocapacitance peak of echinomycin was observed with dsDNA-echinomycin complex, but not with ssDNA-echinomycin comlex. This result is in agreement with the known strong binding of echinomycin to dsDNA by bis-intercalation and only very weak interactin of echinomycin with ssDNA. |
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