Metody stanovení antioxidační aktivity přírodních látek in vitro
| Title in English | In vitro methods for estimation of the antioxidant activity of natural compounds |
|---|---|
| Authors | |
| Year of publication | 2004 |
| Type | Article in Periodical |
| Magazine / Source | Chemické listy |
| MU Faculty or unit | |
| Citation | |
| Field | Biochemistry |
| Keywords | antioxidative activity; free radicals; antioxidants; plant extracts |
| Description | The role of free radicals in pathogenesis of many dysfunctions such as carcinogenesis, cardiovascular diseases and aging is intensively studied. Attention is focused on the contribution of natural antioxidants to protection against the processes. During the last decade, many analytical methods have been developed to determine the antioxidant activity of natural compounds and their mixtures in vitro. This paper reviews the assays used most frequently for this purpose. The procedures are divided into two groups. The first group involves the methods based on the generation of various radical species and their elimination by treatment with antioxidant compounds. The tests used for the evaluation of total antioxidant activity (TEAC, DPPH, ORAC) and the methods testing the ability to prevent lipid peroxidation are included. The other group includes the procedures that are based on measurement of redox properties of compounds (FRAP, CV, HPLC with coulochemical detection). |