Project information
Czech Infrastructure for Integrative Structural Biology
(CIISB)
- Project Identification
- LM2018127
- Project Period
- 1/2020 - 12/2022
- Investor / Pogramme / Project type
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Ministry of Education, Youth and Sports of the CR
- Large Infrastructures for Research, Development and Innovation
- MU Faculty or unit
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Central European Institute of Technology
- prof. RNDr. Vladimír Sklenář, DrSc.
- Cooperating Organization
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Institute of Biotechnology CAS
The Czech Infrastructure for Integrative Structural Biology (CIISB) is a distributed infrastructure of the core facilities and central laboratories of CEITEC (Central European Institute of Technology, Brno) and BIOCEV (Biotechnology and Biomedicine Centre, Vestec) providing expertise and access to technologies used for integrative approaches to structural analysis of biologically important cellular components and macromolecules - proteins, nucleic acids, and their complexes. CIISB, equipped with the cutting-edge technologies, will offer access to the equipment and expertise in the following fields: high-field NMR spectroscopy; high throughput crystallization of biological macromolecules; X-ray diffraction techniques; Bio-SAXS measurements; AFM imaging; characterization of proteins, nucleic acids and complexes by biophysical methods including microcalorimetry, dynamic light scattering, and surface plasmon resonance; high-end cryo-electron microscopy and tomography allowing studies of cellular structures, organelles, and biomacromolecular complexes by 3D imaging; protein expression in eukaryotic cells; high-end mass spectrometry characterization of biomacromolecules; determination of protein post-translational modifications; MS-based peptide mapping/sequencing; high throughput sequencing and proteomic services. CIISB infrastructure aims to provide a high quality open access services in provision of methods and techniques on a level of national and trans-national importance. CIISB is a part of the ESFRI project INSTRUCT-ERIC.
Sustainable Development Goals
Masaryk University is committed to the UN Sustainable Development Goals, which aim to improve the conditions and quality of life on our planet by 2030.
Publications
Total number of publications: 327
2021
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Reproducibility and accuracy of microscale thermophoresis in the NanoTemper Monolith: a multi laboratory benchmark study
European Biophysics Journal With Biophysics Letters, year: 2021, volume: 50, edition: 3-4, DOI
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Revealing structural peculiarities of homopurine GA repetition stuck by i-motif clip
Nucleic Acids Research, year: 2021, volume: 49, edition: 20, DOI
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RNF43 inhibits WNT5A-driven signaling and suppresses melanoma invasion and resistance to the targeted therapy
eLife, year: 2021, volume: 10, edition: October, DOI
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Roles of individual human Dishevelled paralogs in the Wnt signalling pathways
Cellular Signalling, year: 2021, volume: 85, edition: September, DOI
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SP3 Protocol for Proteomic Plant Sample Preparation Prior LC-MS/MS
Frontiers in Plant Science, year: 2021, volume: 12, edition: MAR, DOI
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Staphylococcus epidermidis Phages Transduce Antimicrobial Resistance Plasmids and Mobilize Chromosomal Islands
mSphere, year: 2021, volume: 6, edition: 3, DOI
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Structure of the dihydrolipoamide succinyltransferase (E2) component of the human alpha-ketoglutarate dehydrogenase complex (hKGDHc) revealed by cryo-EM and cross-linking mass spectrometry: Implications for the overall hKGDHc structure
Biochimica et Biophysica Acta - General Subjects, year: 2021, volume: 1865, edition: 6, DOI
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Supramolecular Coronation of Platinum(II) Complexes by Macrocycles: Structure, Relativistic DFT Calculations, and Biological Effects
Inorganic Chemistry, year: 2021, volume: 60, edition: 23, DOI
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Supramolecular hydrogelation via host-guest anion recognition: Lamellar hydrogel materials for the release of cationic cargo
CHEM, year: 2021, volume: 7, edition: 9, DOI
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Synthesis and characterization of WS2/SiO2 microfibers
Journal of Materials Science, year: 2021, volume: 56, edition: 18, DOI