Evaluation of antioxidative activity of flavonoids and exctract of Scutellaria baicalensis with coulochemical detection

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Year of publication 2002
Type Article in Proceedings
Conference Zborník XVIII.Biochemický zjazd
MU Faculty or unit

Faculty of Medicine

Field Biochemistry
Keywords Scutellaria baicalensis; flavonoids; HPLC-EC; antioxidants
Description In the present study, measuring of hydrodynamic voltammograms using HPLC-ECD was used for the estimation of oxidative potentials of five flavonoids of SCB (B,BG, W, WG, O), and two standards (rutin R, quercetin Q). The compounds with lower potentials (below 400 mV) are regarded as strong antioxidants. Among the individual flavonoids of SCB only B a BG displayed significantly lower values of the first oxidative potentials (50, 150 mV resp.). The HPLC-ECD method was then applied on the analysis of crude extracts from SCB. The peak of BG was absolutely dominant in the chromatogram of the extract, indicating that due to high content and low oxidative potential BG is predominantly responsible for the antioxidative effect of the extract. In conclusion, the HPLC-EC presented here is very suitable for screening of antioxidative components in complex mixtures, especially in plant extracts.
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