Hodnocení protinádorového efektu antigen-specifických T lymfocytů s využitím neradioaktivního testu cytotoxity
| Title in English | Evalution of antigen-specific lymphocytes anti-tumor effect with use of non-radioactive cytotoxicity test |
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| Authors | |
| Year of publication | 2006 |
| Type | Article in Periodical |
| Magazine / Source | Transfuze a hematologie dnes |
| MU Faculty or unit | |
| Citation | |
| Field | Oncology and hematology |
| Keywords | cytoxicity test; CFSE; cytotoxic T lymphocytes |
| Description | The optimal stning concentration of CFSE has been found(1mM). Cytotoxicity has been evaluated with effector cells:target cells ratio 2:1,10:1 and 50:1 after 4, 24 and 48 hours of in vitro cultivation. Tumor specificity has been verified by 2 sets of controls: third-party PBMC as negative control target cells and expanded CFSE-labeled interferon gamma negative fraction of T cells as negative control effector cells. |