A device for investigation of natural cell mobility and deformability

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Publikace nespadá pod Lékařskou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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LEDVINA Vojtěch KLEPÁRNÍK Karel LEGARTOVÁ Soňa BÁRTOVÁ Eva

Rok publikování 2020
Druh Článek v odborném periodiku
Časopis / Zdroj Electrophoresis
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www https://onlinelibrary.wiley.com/doi/abs/10.1002/elps.201900357
Doi http://dx.doi.org/10.1002/elps.201900357
Klíčová slova Cell deformability; Cell mobility; Confocal microscopy; Metastasis; Microfluidics
Popis A microfluidic device made of polydimethylsiloxane was developed for continuous evaluation of natural migration mobility of many eukaryotic cells in relaxed and deformed state. The device was fabricated by standard photolithography and soft lithography techniques using the SU-8 3010 negative photoresist on a glass wafer as the master mold. The simple flow-free device exploits the chemotactic movement of cells through a set of mechanical barriers in the direction of concentration gradients of attractants. The barriers are formed by arrays of circular cross-section pillars with decreasing spacing 7, 5, and 3 mu m. To pass through the obstacles, the cells are deformed and change their cytoskeletal architecture. The instantaneous migration velocities of cells are monitored in a time-lapse setup of the scanning confocal microscope. Thus, the cellular deformability and migratory activity can easily be evaluated. The functionality of the device was tested with model HeLa cells stably transfected with fluorescent Premo FUCCI Cell Cycle Sensor. The designed device has the potential to be implemented for testing the tendency of patients’ tumors to metastasis.

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