Structure and mechanism of DNA delivery of a gene transfer agent

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Publikace nespadá pod Lékařskou fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.

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BÁRDY Pavol FÜZIK Tibor HREBÍK Dominik PANTŮČEK Roman THOMAS BEATTY John PLEVKA Pavel

Rok publikování 2020
Druh Článek v odborném periodiku
Časopis / Zdroj Nature Communications
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www https://doi.org/10.1038/s41467-020-16669-9
Doi http://dx.doi.org/10.1038/s41467-020-16669-9
Klíčová slova Alphaproteobacteria; Cryoelectron microscopy; Phage biology; Virus–host interactions; Virus structures; Gene Transfer Agent
Přiložené soubory
Popis Alphaproteobacteria, which are the most abundant microorganisms of temperate oceans, produce phage-like particles called gene transfer agents (GTAs) that mediate lateral gene exchange. However, the mechanism by which GTAs deliver DNA into cells is unknown. Here we present the structure of the GTA of Rhodobacter capsulatus (RcGTA) and describe the conformational changes required for its DNA ejection. The structure of RcGTA resembles that of a tailed phage, but it has an oblate head shortened in the direction of the tail axis, which limits its packaging capacity to less than 4,500 base pairs of linear double-stranded DNA. The tail channel of RcGTA contains a trimer of proteins that possess features of both tape measure proteins of long-tailed phages from the family Siphoviridae and tail needle proteins of short-tailed phages from the family Podoviridae. The opening of a constriction within the RcGTA baseplate enables the ejection of DNA into bacterial periplasm.
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