Biocompatibility of a genetically encoded calcium indicator in a transgenic mouse model

Varování

Publikace nespadá pod Lékařskou fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.

Autoři	DIRENBERGER Tephan MUES Marsilius MICALE Vincenzo WOTJAK Carsten T DIETZEL Steffen SCHUBERT Michael SCHARR Andreas HASSAN Sami WAHL-SCHOTT Christian BIEL Martin KRISHNAMOORTHY Gurumoorthy GRIESBECK Oliver
Rok publikování	2012
Druh	Článek v odborném periodiku
Časopis / Zdroj	NATURE COMMUNICATIONS
Fakulta / Pracoviště MU	Středoevropský technologický institut
Citace
www	http://www.nature.com/ncomms/journal/v3/n8/pdf/ncomms2035.pdf?WT.ec_id=NCOMMS-20120828
Doi	http://dx.doi.org/10.1038/ncomms2035
Obor	Neurologie, neurochirurgie, neurovědy
Klíčová slova	GREEN FLUORESCENT PROTEIN; TROPONIN-C; IN-VIVO; SINOATRIAL NODE; RESISTANCE ARTERIES; CA2+ INDICATORS; RHOA/RHO-KINASE; MICE; EXPRESSION; ACTIVATION
Popis	Engineering efforts of genetically encoded calcium indicators predominantly focused on enhancing fluorescence changes, but how indicator expression affects the physiology of host organisms is often overlooked. Here, we demonstrate biocompatibility and widespread functional expression of the genetically encoded calcium indicator TN-XXL in a transgenic mouse model. To validate the model and characterize potential effects of indicator expression we assessed both indicator function and a variety of host parameters, such as anatomy, physiology, behaviour and gene expression profiles in these mice. We also demonstrate the usefulness of primary cells and organ explants prepared from these mice for imaging applications. Although we find mild signatures of indicator expression that may be further reduced in future sensor generations, the 'green' indicator mice generated provide a well-characterized resource of primary cells and tissues for in vitro and in vivo calcium imaging applications.