Cytotoxic Constituents from Lobaria scrobiculata and a Comparison of Two Bioassays for Their Evaluation

Autoři	SCHINKOVITZ Andreas KANG Avinash Kaur URBAN Ernst ZEHL Martin PÁCHNIKOVÁ Gabriela WANG Yuehong KRETSCHMER Nadine SLANINOVÁ Iva PAULI Guido F. FRANZBLAU Scott G. KRUPITZA Georg BAUER Rudolf KOPP Brigitte
Rok publikování	2014
Druh	Článek v odborném periodiku
Časopis / Zdroj	Journal of Natural Products
Fakulta / Pracoviště MU	Lékařská fakulta
Citace
Doi	https://doi.org/10.1021/np4008574
Obor	Farmakologie a lékárnická chemie
Klíčová slova	USNEA-LONGISSIMA; LICHEN; ACID; PULMONARIA
Popis	Lichens are resilient organisms, known for their unique profiles of secondary metabolites and for exhibiting antioxidative, antibacterial, and cytotoxic effects. Analyzing the cytotoxic potential of Lobaria scrobiculata, a bioassay-guided fractionation strategy yielded seven known metabolites, with two of these compounds, 2 and 3, exhibiting cytotoxicity against HL-60 cells. In order to verify the potential impact of degradation on observed bioactivity, a purity and stability evaluation was conducted. The consistency of results obtained by the water-soluble tetrazolium salt-1 assay and trypan blue cytotoxicity assay was evaluated for selected compounds.