Chloroacetamide-Linked Nucleotides and DNA for Cross-Linking with Peptides and Proteins

Varování

Publikace nespadá pod Lékařskou fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.

Autoři	OLSZEWSKA Agata POHL Radek BRAZDOVA Marie FOJTA Miroslav HOCEK Michal
Rok publikování	2016
Druh	Článek v odborném periodiku
Časopis / Zdroj	BIOCONJUGATE CHEMISTRY
Fakulta / Pracoviště MU	Středoevropský technologický institut
Citace
www	http://pubs.acs.org/doi/abs/10.1021/acs.bioconjchem.6b00342
Doi	http://dx.doi.org/10.1021/acs.bioconjchem.6b00342
Obor	Biochemie
Klíčová slova	TUMOR-SUPPRESSOR P53; DIELS-ALDER REACTION; REDUCTIVE AMINATION; ENZYMATIC-SYNTHESIS; LYSINE RESIDUES; CLICK CHEMISTRY; BINDING; TRIPHOSPHATES; PROBES; AZIDE
Popis	Nucleotides, 2'-deoxyribonucleoside triphosphates (dNTPs), and DNA probes bearing reactive chloroacetamido group linked to nucleobase (cytosine or 7-deazadaenine) through a propargyl tether were prepared and tested in cross-linking with cysteine- or histidine-containing peptides and proteins. The chloroacetamide-modifed dNTPs proved to be good substrates for DNA polymerases in the enzymatic synthesis of modified DNA probes. Modified nucleotides and DNA reacted efficiently with cysteine and cysteine-containing peptides, whereas the reaction with histidine was sluggish and low yielding. The modified DNA efficiently cross-linked with p53 protein through alkylation of cysteine and showed potential for cross-linking with histidine (in C277H mutant of p53).
Související projekty:	Centrum nových přístupů k bioanalýze a molekulární diagnostice