Time- and Dose-Dependent Effects of 17 Beta-Estradiol on Short-Term, Real-Time Proliferation and Gene Expression in Porcine Granulosa Cells

Autoři	CIESIÓŁKA Sylwia BUDNA Joanna JOPEK Karol BRYJA Artur KRANC Wiesława BORYS Sylwia JEŠETA Michal CHACHUŁA Adrian ZIÓŁKOWSKA Agnieszka ANTOSIK Paweł BUKOWSKA Dorota BRÜSSOW Klaus P. BRUSKA Małgorzata NOWICKI Michał ZABEL Maciej KEMPISTY Bartosz
Rok publikování	2017
Druh	Článek v odborném periodiku
Časopis / Zdroj	Biomed Research International
Fakulta / Pracoviště MU	Lékařská fakulta
Citace
Doi	http://dx.doi.org/10.1155/2017/9738640
Obor	Biotechnologie a bionika
Klíčová slova	CELLULAR-DISTRIBUTION; CUMULUS CELLS; FOLLICULAR DEVELOPMENT; PROTEIN EXPRESSION; OOCYTE; PROGESTERONE; ESTROGEN; CONNEXIN-43; COMPETENCE; CX43
Popis	The key mechanisms responsible for achievement of full reproductive and developmental capability in mammals are the differentiation and transformation of granulosa cells (GCs) during folliculogenesis, oogenesis, and oocytematuration. Although the role of 17 beta-estradiol (E2) in ovarian activity is widely known, its effect on proliferative capacity, gap junction connection (GJC) formation, andGCs-luteal cells transformation requires further research. Therefore, the goal of this study was to assess the real-time proliferative activity of porcine GCs in vitro in relation to connexin (Cx), luteinizing hormone receptor (LHR), follicle stimulating hormone receptor (FSHR), and aromatase (CYP19A1) expression during short-term(168 h) primary culture. The cultured GCs were exposed to acute (at 96 h of culture) and/or prolonged (between 0 and 168 h of culture) administration of 1.8 and 3.6.. mu M E2. The relative abundance of Cx36, Cx37, Cx40, Cx43, LHR, FSHR, and CYP19A1mRNA wasmeasured. We conclude that the proliferation capability of GCs in vitro is substantially associated with expression of Cxs, LHR, FSHR, andCYP19A1. Furthermore, the GC-luteal cell transformation in vitro may be significantly accompanied by the proliferative activity of GCs in pigs.