Local chemical sympathectomy of rat bone marrow and its effect on marrow cell composition

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DUBOVÝ Petr KLUSÁKOVÁ Ilona KUČERA Lukáš OSIČKOVÁ Jitka CHOVANCOVÁ Jana LOJA Tomáš MAYER Jiří DOUBEK Michael JOUKAL Marek

Rok publikování 2017
Druh Článek v odborném periodiku
Časopis / Zdroj AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
www https://doi.org/10.1016/j.autneu.2017.06.003
Doi http://dx.doi.org/10.1016/j.autneu.2017.06.003
Obor Morfologické obory a cytologie
Klíčová slova Guanethidine; Osmotic mini pump; Sympathetic innervation; Endothelial cells
Popis Existing experimental studies of the effect of sympathetic nerve fibers on bone marrow cells are based on the systemic administration of neurotoxic 6-hydroxydopamine. The method of global chemical sympathectomy has some serious disadvantages and could lead to questionable results. We describe a new method of local chemical sympathectomy of rat femoral bone marrow using guanethidine (Ismelin) delivery using an osmotic mini pump. Local guanethidine treatment for 14 days led to complete elimination of sympathetic fibers in femoral bone marrow in contrast to bone marrow of contralateral or naive femurs. Ablation of sympathetic fibers was associated with a loss of rat endothelial cell marker (RECA) indicating immunophenotype changes in blood vessel endothelial cells, but no significant effect of guanethidine was found on the survival of endothelial cells and mesenchymal stem cells in vitro. Moreover, local guanethidine treatment also elicited a significant reduction of Nestin +/SDF1 + mesenchymal stem cells and c-Kit +/CD90 + hematopoietic stem cells in femoral bone marrow. Tissue-specific chemical sympathectomy of rat bone marrow by guanethidine overcomes some of the drawbacks of systemic administration of neurotoxic compounds like 6-hydroxydopamine and delivers unequivocal evidence on the effects of sympathetic innervation on the cell content of bone marrow.
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