Generation of three human iPSC lines from patients with a spontaneous late-onset Alzheimer's disease and three sex- and age-matched healthy controls

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RAŠKA Jan KLÍMOVÁ Hana SHEARDOVÁ Kateřina FEDOROVÁ Veronika HŘÍBKOVÁ Hana POSPÍŠILOVÁ Veronika VOCHYÁNOVÁ Simona VÁŇOVÁ Tereza BOHAČIAKOVÁ Dáša

Rok publikování 2021
Druh Článek v odborném periodiku
Časopis / Zdroj Stem cell research
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
www https://www.sciencedirect.com/science/article/pii/S1873506121002245?via%3Dihub
Doi http://dx.doi.org/10.1016/j.scr.2021.102378
Klíčová slova human iPSC lines; spontaneous late-onset Alzheimer’s disease
Popis Human induced pluripotent stem cell (iPSC) lines were generated from patients with spontaneous late-onset Alzheimer's disease (AD) and three healthy control individuals. Peripheral blood mononuclear cells were reprogrammed with Yamanaka factors (OSKM) using a commercially available Epi5 Reprogramming Kit. The pluripotency of iPSCs was confirmed by the expression of pluripotency factors and by their ability to differentiate to all three germ layers in vitro. Newly derived cell lines can be used to model Alzheimer's disease in vitro.
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