A study of the effect of DAMPs on choroid plexus using an in-vitro model



Rok publikování 2021
Druh Další prezentace na konferencích
Popis In case of tissue injury, damage-associated molecular patterns (DAMPs) are released into the blood circulation. DAMPs might migrate through the blood-cerebrospinal fluid barrier (BCSF-B) and trigger toll-like receptors (TLRs) in the choroid plexus, which can potentially result in the immune reaction in the central nervous system. To study this mechanism an in-vitro model of choroid plexus (Z310 cells) was used and the proinflammatory reaction of choroidal epithelial cells in response to DAMPs was examined. Z310 cells represent primary choroidal epithelial cell culture established from rat choroid plexus tissue. Z310 cells were incubated with the pattern recognition receptors (PRRs) agonists, such as LPS (lipopolysaccharide), N-Formylated Peptide (fmlp), and CpG oligodeoxynucleotides (CPG ODN) for 24 hours. Cells were then used for immunocytochemistry and western blot analyses of expression of TLR4, TLR9, and cytokines IL-6, IL1-ß, TNF-?. The expression of cytokines IL-6, IL1-ß, TNF-? were upregulated in Z310 cells, following activation of PRRs via DAMPs. After tissue injury, DAMPs can migrate through BSCF-B and they have the ability to increase the pro-inflammatory profiling of the choroidal epithelial cells.
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