Increased occurrence of Treponema spp. and double-species infections in patients with Alzheimer's disease

Varování

Publikace nespadá pod Lékařskou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.

Autoři	NEMERGUT Michal BÁŤKOVÁ Tereza VIGAŠOVÁ Dana BARTOŠ Milan HLOZANKOVA Martina SMITH Andrea LIŠKOVÁ Barbora SHEARDOVÁ Kateřina VYHNALEK Martin HORT Jakub LACZO Jan KOVÁČOVÁ Ingrid ŠITINA Michal MATEJ Radoslav JANČÁLEK Radim MAREK Martin DAMBORSKÝ Jiří
Rok publikování	2022
Druh	Článek v odborném periodiku
Časopis / Zdroj	Science of the Total Environment
Fakulta / Pracoviště MU	Přírodovědecká fakulta
Citace
www	https://www.sciencedirect.com/science/article/pii/S0048969722042115?via%3Dihub
Doi	http://dx.doi.org/10.1016/j.scitotenv.2022.157114
Klíčová slova	Alzheimer'sdisease; Treponemaspp; Microbialinfection; Pathogen; Serum; PCR
Popis	Although the link between microbial infections and Alzheimer's disease (AD) has been demonstrated in multiple studies, the involvement of pathogens in the development of AD remains unclear. Here, we investigated the fre-quency of the 10 most commonly cited viral (HSV-1, EBV, HHV-6, HHV-7, and CMV) and bacterial (Chlamydia pneumoniae, Helicobacter pylori, Borrelia burgdorferi, Porphyromonas gingivalis, and Treponema spp.) pathogens in serum, cerebrospinal fluid (CSF) and brain tissues of AD patients. We have used an in-house multiplex PCR kit for simultaneous detection of five bacterial and five viral pathogens in serum and CSF samples from 50 AD patients and 53 healthy controls (CTRL). We observed a significantly higher frequency rate of AD patients who tested positive for Treponema spp. compared to controls (AD: 62.2 %; CTRL: 30.3 %; p-value = 0.007). Fur-thermore, we confirmed a significantly higher occurrence of cases with two or more simultaneous infections in AD patients compared to controls (AD: 24 %; CTRL 7.5 %; p-value = 0.029). The studied pathogens were de-tected with comparable frequency in serum and CSF. In contrast, Borrelia burgdorferi, human herpesvirus 7, and human cytomegalovirus were not detected in any of the studied samples. This study provides further evidence of the association between microbial infections and AD and shows that paralleled analysis of multiple sample specimens provides complementary information and is advisable for future studies.
Související projekty:	Personalizovaná medicína - diagnostika a terapie Decoding the molecular principles of enzyme evolution Klinicky relevantní biochemické, imunologické a buněčné biomarkery Alzheimerovy nemoci a stárnutí