Carp edema virus infection associated gill pathobiome: A case report

Varování

Publikace nespadá pod Lékařskou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.

Autoři	PALÍKOVÁ Miroslava POJEZDAL Ľubomír DÁVIDOVÁ-GERŽOVÁ Lenka NOVÁKOVÁ Věra PIKULA Jiří PAPEŽÍKOVÁ Ivana MINÁŘOVÁ Hana MIKULÍKOVÁ Ivana DYKOVÁ Iva
Rok publikování	2022
Druh	Článek v odborném periodiku
Časopis / Zdroj	Journal of Fish Diseases
Fakulta / Pracoviště MU	Přírodovědecká fakulta
Citace
www	https://doi.org/10.1111/jfd.13670
Doi	http://dx.doi.org/10.1111/jfd.13670
Klíčová slova	CEV; Cyprinus carpio; fish; pathogenic assemblage; virus diseases
Popis	Understanding disease aetiology and pathologic mechanisms is essential for fish health evaluation. Carp edema virus (CEV) is the causative agent of a disease (CEVD) responsible for high mortality rates in both wild and cultured common carp Cyprinus carpio. Inspection of two carp specimens from a pond with high fish mortality revealed CEV infection in both the host and its ectoparasite (Argulus foliaceus). In addition to flavobacteria, well known to be associated with gill lesions, we found that free-living eukaryotes (amoebae and ciliates) and a temporary parasite (Ichthyobodo spp.) colonizing the gills may also contribute to alterations in gill structure and/or function, either directly, through firm (Ichthyobodo) or weak (amoebae) attachment of trophozoites to the gill epithelium, or indirectly, through carriage of pathogenic bacteria. Bacterial assemblages rich in families and genera, with predominance of Cetobacterium spp. in low-intensity alteration of the gill tissue and of Flavobacterium spp. in gills with extensive necrotic lesions, were detected in gills and within the cytoplasm of associated amoebae using high-throughput sequencing. Quantitative PCR indicated F. swingsii as the prevailing flavobacterial species within amoebae from less affected gills and F. psychrophilum within amoebae from extensively affected gills. This case study suggests that eukaryotic organisms as part of the gill pathobiome may also contribute to irreversible gill lesions seen in CEVD. Emphasizing the complexity of mutual relationships between bacterial assemblages and eukaryotic co-pathogens, further studies regarding factors that trigger pathology and influence severity in the CEV-positive carp are needed.