Apoptosis in pig leucocytes during cardiopulmonary bypass

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Publikace nespadá pod Lékařskou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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MATALOVÁ Eva ŠPANOVÁ Alena RITTICH Bohuslav WAGNER Robert KOVÁŘŮ František

Rok publikování 1999
Druh Článek ve sborníku
Konference Inflammatory Paradigms and the Vasculature
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Obor Genetika a molekulární biologie
Popis The aim of this study was to observe programmed cell death (PCD) activation in pig leucocytes durind cardiopulmonary bypass (CPB). Blood samples were taken prior to, during, and after CPB. Apoptosis was detected using TUNEL assay and by typical apoptotic morphology changes (Giemsa staining). Apoptotic DNA fragmentation was proved in high percentage of cells in the operating beginning. A decrease in the number of apoptotic cells was detected in the surviving pigs after the aorta clip release. On the other hand, the number of apoptotic leucocytes did not significantly change in pigs died owing to postperfusion syndrom. The same alterations in percentage of apoptotic cells were proved in bone marrow after chest opening, after surgery and in dead animals. These results suggested that PCD can play a role in the postperfusion syndrome after CPB.
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