Retron reverse transcriptase rrtT is ubiquitous in strains of Salmonella enterica serovar Typhimurium

Varování

Publikace nespadá pod Lékařskou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.

Autoři	MATIASOVIČOVÁ Jitka FALDYNOVÁ Marcela PRAVCOVÁ Martina KARPÍŠKOVÁ Renata KOLÁČKOVÁ Ivana DAMBORSKÝ Jiří RYCHLÍK Ivan
Rok publikování	2003
Druh	Článek v odborném periodiku
Časopis / Zdroj	FEMS Microbiology Letters
Fakulta / Pracoviště MU	Přírodovědecká fakulta
Citace
www	http://ncbr.chemi.muni.cz/~jiri/abstracts/femslett03.html
Obor	Mikrobiologie, virologie
Klíčová slova	RRTT; TYPHYMURIUM; SALMONELLA; ENTERICA
Popis	Bacterial retron reverse transcriptases are unusual enzymes which utilise the same RNA molecule as a template and also as a primer for initiation of the reverse transcription. Except for their relatively frequent presence in Myxococcus spp., they are considered as quite rare proteins. However, in this study we proved that retron reverse transcriptase is frequently found in certain serovars of Salmonella enterica. Using PCR, in strains of serovar Typhimurium, the rrtT (retron reverse transcriptase Typhimurium) gene was detected in 158 out of 175 tested field strains. On the other hand, in none of the 18 tested serovar Enteritidis strains the rrtT was detected in their genome. Detailed computer analysis allowed us to predict the sequence of msDNA and to propose that the final msDNA is free of any RNA. Furthermore, we predict that there are at least three different classes of retron reverse transcriptases.
Související projekty:	Biomolekulární centrum