Microtubules and actin cytoskeleton of potentially pathogenic basidimycetous yeast as target for antifungals
| Autoři | |
|---|---|
| Rok publikování | 2009 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | CHEMOTHERAPY |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | |
| Obor | Mikrobiologie, virologie |
| Klíčová slova | Yeast;Actin;Microtubules;Paclitaxel;Vincristine;Vinblastine;Methyl benzimidazole2yl carbamate;Thiabendazole; Cytochalasins A B D; Latrunculin A |
| Popis | The cytoskeleton was investigated as a potential target for the inhibition of cell division in Fellomyces fuzhou-ensis related to Cryptococcus neoformans.Vincristine, vinblastine,paclitaxel,methyl benzimidazole-2-yl carbamate (BCM),thiabendazole,cytochalasins A,B,D and latrunculin A were added to cells and investigated.Vincristine, vinblastine, paclitaxel, cytochalasins A,B and D transiently blocked proliferation. BCM disrupted microtubules,inhibited mitosis, but F-actin structures persisted and neck-less conidia originated. Latrunculin disrupted F-actin, cells became spherical,stalks and necks degenerated, microtubules persisted,mitosis,cytokinesis and conidiogenesis were blocked.The combined application of latrunculin and BCM disrupted F-actin and microtubules,inhibited cells became spherical and did not divide.Conclusion: Microtubules and F-actin are effective targets for permanent inhibition of nuclear and cell division and conidiogenesis by BCM and latrunculin A. |
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