Ultrastructural disorder of the secretory pathway in temperature-sensitive actin mutants of Saccharomyces cerevisiae
| Autoři | |
|---|---|
| Rok publikování | 2010 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Journal of Electron Microscopy |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | http://opac.ndl.go.jp:80/recordid/000000129291/eng |
| Doi | http://dx.doi.org/10.1093/jmicro/dfp050 |
| Obor | Ostatní lékařské obory |
| Klíčová slova | actin mutant; freeze-fracturing; freeze-substitution; secretory pathway; Golgi apparatus |
| Popis | Phenotypes of the two temperature-sensitive actin mutants of Saccharomyces cerevisiae act1-1 and act1-2 at permissive, restrictive and semirestrictive temperatures were studied by freeze fracture and thin section electron micr., and fluoresc. micr. In contrast to secretory mutants where accumulations of either secretory vesicles, Golgi app., or end. reticulum were reported, act1-1 and act1-2 mutants revealed accumulation of all the three components, even at 23C. However, more distinct accumulation of secretory organelles was evident during cultivation at the sub-restrictive temperature of 30C. At temperature of 37C, many cells died, and their empty cell walls remained. Some of the few living cells showed features of apoptosis. From the present study, actin cables are concluded to be necessary for (i) correct spatial positioning and orientation of secretary pathway to the bud and septum, and (ii) vectorial movement of vesicles of the secretory pathway along the actin cables to the bud and septum. |
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