Artificial Neural Networks for Classification in Metabolomic Studies of Whole Cells Using H-1 Nuclear Magnetic Resonance

• Autoři: BROUGHAM D F; IVANOVA G.; GOTTSCHALK M.; COLLINS D M; EUSTACE A J; O CONNOR R.; HAVEL Josef
• Rok publikování: 2011
• Druh: Článek v odborném periodiku
• Časopis / Zdroj: Journal of Biomedicine and Biotechnology
• Fakulta / Pracoviště MU: Přírodovědecká fakulta
• Doi: http://dx.doi.org/10.1155/2011/158094
• Obor: Analytická chemie, separace
• Klíčová slova: NMR SPECTROSCOPIC DATA; HUMAN BRAIN-TUMORS; H-1-NMR SPECTROSCOPY; PATTERN-RECOGNITION; IN-VITRO; EXTRACTS; IDENTIFICATION; LINES; VIVO; RESISTANCE
• Popis: We report the successful classification, by artificial neural networks (ANNs), of H-1 NMR spectroscopic data recorded on whole-cell culture samples of four different lung carcinoma cell lines, which display different drug resistance patterns. The robustness of the approach was demonstrated by its ability to classify the cell line correctly in 100% of cases, despite the demonstrated presence of operator-induced sources of variation, and irrespective of which spectra are used for training and for validation. The study demonstrates the potential of ANN for lung carcinoma classification in realistic situations.

Související projekty

• Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu