The ATRA-induced differentiation of medulloblastoma cells is enhanced with LOX/COX inhibitors: an analysis of gene expression.

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CHLAPEK Petr NERADIL Jakub LOJOVÁ Martina ZITTERBART Karel ŠTĚRBA Jaroslav VESELSKÁ Renata

Rok publikování 2014
Druh Článek v odborném periodiku
Časopis / Zdroj Cancer Cell International
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www http://www.cancerci.com/content/14/1/51
Doi http://dx.doi.org/10.1186/1475-2867-14-51
Obor Genetika a molekulární biologie
Klíčová slova all-trans retinoic acid; caffeic acid; celecoxib; medullobastoma; LOX and COX inhibitors
Popis A detailed analysis of the expression of 440 cancer-related genes was performed after the combined treatment of medulloblastoma cells with all-trans retinoic acid (ATRA) and inhibitors of lipoxygenases (LOX) and cyclooxygenases (COX). The Daoy and D283 Med medulloblastoma cell lines were chosen for this study. Caffeic acid (an inhibitor of 5-LOX) and celecoxib (an inhibitor on COX-2) were used in combined treatment with ATRA. The results clearly confirmed that the differentiating effects of ATRA should be enhanced via its combined administration with caffeic acid or celecoxib. An increased expression of the genes that encoded the proteins participating in induced differentiation and cytoskeleton remodeling was detected in both cell lines in a concentration-dependent manner. This effect was also observed for the CDKN1A gene encoding the p21 protein, which is an important regulator of the cell cycle, and for the genes encoding proteins that are associated with proteasome activity. Furthermore, our results showed that D283 Med cells are significantly more sensitive to treatment with ATRA alone than Daoy cells.
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