In Vivo Reporters for Visualizing Alternative Splicing of Hormonal Genes

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Publikace nespadá pod Lékařskou fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
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KASHKAN Ivan TIMOFEYENKO Kseniya ŠLIKOVÁ Eva RŮŽIČKA Kamil

Rok publikování 2020
Druh Článek v odborném periodiku
Časopis / Zdroj Plants
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www https://www.mdpi.com/2223-7747/9/7/868
Doi http://dx.doi.org/10.3390/plants9070868
Klíčová slova alternative splicing; auxin; cytokinins; brassinosteroids; AHP6; DWF4; PIN7; D-amino acid oxidase; genetic screen; Arabidopsis
Popis Rapid progress in plant molecular biology in recent years has uncovered the main players in hormonal pathways and characterized transcriptomic networks associated with hormonal response. However, the role of RNA processing, in particular alternative splicing (AS), remains largely unexplored. Here, using example genes involved in cytokinin signaling, brassinosteroid synthesis and auxin transport, we present a set of reporters devised to visualize their AS events in vivo. These reporters show a differential tissue-specific expression of certain transcripts and reveal that expression of some of the them can be changed by the application of the exogenous hormone. Finally, based on the characterized AS event of the PIN7 auxin efflux carrier, we designed a system that allows a rapid genetic screening for the factors upstream of this AS event. Our innovative toolset can be therefore highly useful for exploring novel regulatory nodes of hormonal pathways and potentially helpful for plant researchers focusing on developmental aspects of AS.
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