Pseudomonas petrae sp. nov. isolated from regolith samples in Antarctica

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Publikace nespadá pod Lékařskou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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NOVÁKOVÁ Dana KOUBLOVÁ Vendula SEDLÁŘ Karel STAŇKOVÁ Eva KRÁLOVÁ Stanislava ŠVEC Pavel NEUMANN-SCHAAL Meina WOLF Jacqueline KOUDELKOVÁ Sylva BARTÁK Miloš SEDLÁČEK Ivo

Rok publikování 2023
Druh Článek v odborném periodiku
Časopis / Zdroj Systematic and Applied Microbiology
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www https://doi.org/10.1016/j.syapm.2023.126424
Doi http://dx.doi.org/10.1016/j.syapm.2023.126424
Klíčová slova Pseudomonas; P. petrae sp. nov.; Antarctica; Regolith; Species description; Taxonomy
Popis A polyphasic taxonomic approach was used to characterize the four strains P2653T, P2652, P2498, and P2647, isolated from Antarctic regolith samples. Initial genotype screening performed by PCR fingerprinting based on repetitive sequences showed that the isolates studied formed a coherent cluster separated from the other Pseudomonas species. Identification results based on 16S rRNA gene sequences showed the highest sequence similarity with Pseudomonas graminis (99.7%), which was confirmed by multilocus sequence analysis using the rpoB, rpoD, and gyrB genes. Genome sequence comparison of P2653T with the most related P. graminis type strain DSM 11363T revealed an average nucleotide identity of 92.1% and a digital DNA-DNA hybridization value of 46.6%. The major fatty acids for all Antarctic strains were C16:0, Summed Feature 3 (C16:1 ?7c/C16:1 ?6c) and Summed Feature 8 (C18:1 ?7c/C18:1 ?6c). The predominant respiratory quinone was Q-9, and the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, and phosphatidylglycerol. The regolith strains could be differentiated from related species by the absence of arginine dihydrolase, ornithine and lysine decarboxylase and by negative tyrosine hydrolysis. The results of this polyphasic study allowed the genotypic and phenotypic differentiation of four analysed strains from the closest related species, which confirmed that the strains represent a novel species within the genus Pseudomonas, for which the name Pseudomonas petrae sp. nov. is proposed with P2653T (CCM 8850T = DSM 112068T = LMG 30619T) as the type strain.
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