Advances in high-resolution cytometry of FISH dots in interphase cell nuclei

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Publikace nespadá pod Lékařskou fakultu, ale pod Fakultu informatiky. Oficiální stránka publikace je na webu muni.cz.
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KOZUBEK Michal KOZUBEK Stanislav LUKÁŠOVÁ Emilie BÁRTOVÁ Eva SKALNÍKOVÁ Magdalena MATULA Pavel MATULA Petr JIRSOVÁ Pavla GAŇOVÁ Alena KOUTNÁ Irena

Rok publikování 2000
Druh Konferenční abstrakty
Fakulta / Pracoviště MU

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Popis Recently, we have described a high-resolution cytometry (HRCM) technique (Cytometry 36:279-293) as a reasonable compromise between flow cytometry (FCM) and laser scanning cytometry (LSCM) on one hand and confocal laser scanning microscopy (CLSM) on the other hand. Whereas FCM/LSCM and CLSM enable measuring of either large number of cells with low resolution or small number of cells with high resolution, respectively, HRCM can analyze quantities comparable to FCM/LSCM measurements (using overnight acquisition) with an accuracy comparable to CLSM. Very recently we have built a second HRCM instrument (see picture at http://www.fi.muni.cz/lom) which enables switching between conventional and confocal modes. The confocal mode is realized using a 5% Nipkow disk optimized for fluorescence imaging. It offers a better resolution but at a lower speed. The user can choose which fluorochromes are imaged in confocal mode. Thus, the system can be configured for a variety of imaging modes ranging from quick 2-D analysis up to high-resolution confocal 3-D studies.
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