Electrochemical Detection of Interactions of Echinomycin with DNA at Mercury Film Electrodes (MFE).
| Autoři | |
|---|---|
| Rok publikování | 2001 |
| Druh | Článek ve sborníku |
| Konference | Book of Abstracts, US-CZ Workshops on Electrochemical Sensors Prague 2001 |
| Fakulta / Pracoviště MU | |
| Citace | |
| Obor | Biofyzika |
| Klíčová slova | DNA-echinomycin interaction; electrochemical impedance spectroscopy;EIS; DNA adsorption; mercuiry film electrodes; |
| Popis | The dependence of the impedance Z and/or differential capacitance C of the electrode double layer on electrode potential E and on frequency in solutions of echinomycin with single stranded (ss) and double-helical (double stranded, ds) DNA was measured. Echinomycin gives a pseudocapacitance redox peak on the C-E curves at the potential of -0.53 V. This peak is observed with the dsDNA-echinomycin comlex as well, but not with ssDNA treated by echinomycin. The capacitance measurements can thus distinguish with the aid of bis-intercalator echinomycin the ss and ds form of DNA adsorbed at the electrode surface. This promise to use impedance measurements for detection of dsDNA in hybridization reactins. |
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