The appearance of truncated cyclin A2 correlates with differentiation of mouse embryonic stem cells

Varování

Publikace nespadá pod Lékařskou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.

Autoři	ANGER M. BRYJA V. JIRMANOVÁ L. HAMPL Aleš MOTLÍK J. DVOŘÁK P. KUBELKA M.
Rok publikování	2003
Druh	Článek v odborném periodiku
Časopis / Zdroj	Biochemical and biophysical research communications
Fakulta / Pracoviště MU	Přírodovědecká fakulta
Citace
Obor	Genetika a molekulární biologie
Klíčová slova	stem cells
Popis	The presence of a form of cyclin A2 with an N-terminal truncation has recently been reported in various murine cell lines and tissues. The truncated cyclin A2 binds to and activates the cyclin-dependent kinase 2 (CDK2). However, CDK2 bound by the truncated cyclin A2 is located in the cytoplasm in contrast to CDK2 bound to full-length cyclin A2, which is in the nucleus. Here, we show that proliferating mouse embryonic stem cells (ES cells) contain very little truncated cyclin A2 but as the cells are induced to differentiate the amount of truncated cyclin A2 increases. The expression pattern of truncated cyclin A2 was the same in p27(Kip1) -/- differentiating ES cells as in the differentiating wild-type cells. We conclude that p27(Kip1) is not necessary for the proteolytic cleavage that gives rise to the truncated form of cyclin A2 in differentiating ES cells and that this post-translational modification is not a function of the cell density but is correlated with differentiation.