Capillary electrophoresis as a member of the metabolomic analytical toolbox (CE metabolomics)

Logo poskytovatele
Kód projektu
GAP206/11/0009
Období řešení
1/2011 - 12/2015
Investor / Programový rámec / typ projektu
Grantová agentura ČR
Fakulta / Pracoviště MU
Přírodovědecká fakulta
Další fakulta/pracoviště MU
Lékařská fakulta

The generic methods for metabolomic studies based on application of different CE modes - capillary zone electrophoresis (CZE), micellar electrokinetic capillary chromatography (MEKC), microemulsion electrokinetic capillary chromatography (MEEKC), nonaqueous capillary electrophoresis (NACE) for corresponding metabolite classes will be developed. The relevant on-line preconcentration strategies will be integrated in given methods and possibility of using with MS detection will be tested. They can be used for metabolite fingerprinting and footprinting, metabolite profiling as well as target analysis. The complete procedure for the target metabolome analysis of microbial samples thus will be available starting from the rapid metabolism quenching and the quantitative extraction of selected metabolites up to their analysis by different CE modes. Besides the new method for the embryo viability testing based on non-invasive metabolite footprinting and profiling will be available as well.

Publikace

Počet publikací: 46


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